In Vitro Growth of Preantral Follicles Isolated from Cryopreserved Ovine Ovarian Tissue
نویسندگان
چکیده
منابع مشابه
Activin promotes oocyte development in ovine preantral follicles in vitro
Activins have been implicated as important regulating factors for many reproductive processes. The aim of this study was to determine the effect of activin A on the development of ovine preantral follicles in vitro. Mechanically isolated preantral follicles (161 +/- 2 microm) were cultured for 6 days in the presence of human recombinant activin A (0, 10 and 100 ng/ml). Half of the medium was re...
متن کاملViability of primordial follicles derived from cryopreserved ovine ovarian cortex tissue.
OBJECTIVE To develop an efficient technique to preserve primordial follicles from cryopreserved ovarian tissue. DESIGN Frozen-thawed and fresh preantral follicles were mechanically isolated for viability testing, and their morphology was histologically analyzed. SETTING Laboratory of Animal Reproduction, Faculty of Veterinary Medicine and Zootechny, University State of São Paulo, Brazil. ...
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Objectives: Evaluating the addition effect of J. insularis extract and FSH on the survival, activation and ROS production after in vitro culture of ovine preantral follicles enclosed in ovarian tissue. Methods: In the first experiment, ovarian fragments were fixed (non-cultured control) or in vitro cultured in α-MEM (cultured control), α-MEM supplemented with FSH 50 ng/mL, or in α-MEM supplemen...
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Purpose: The aim of this study was to investigate the growth and survival rate of preantral follicles isolated from vitrified ovarian tissue by cryotop.Materials and Methods: Ovaries of 14-day-old NMRI mice were separated and divided into three groups fowling as: vitrified, toxicity tested and control groups. Ovaries in the experimental groups were immersed in equilibration and vitrification so...
متن کاملLive offspring from cryopreserved embryos following in vitro growth, maturation and fertilization of oocytes derived from preantral follicles in mice.
This study was undertaken to examine pre- and postimplantation developmental potency of cryopreserved embryos that had undergone in vitro growth (IVG), maturation (IVM) and fertilization (IVF) of oocytes from the preantral follicle stage. An oocyte culture system for IVG and IVM was used in oocyte-granulosa cell complexes (OGCs) derived from preantral follicles in 12-day-old mice. The rate of o...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 2004
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod.103.016774